With the increase of tolerance of winter resistance in Pinus bungeana seedlings, its antioxidation enzyme recovery system ( SOD, CAT, POD) activity was increased obviously. 白皮松幼苗抗寒性提高后体内抗氧化酶促防御系统(SOD、CAT、POD)活性明显提高,而且3种酶活性变化之间的相互匹配是提高白皮松幼苗抗寒性的关键因素;
Methods: Ultrasonic, DNA enzyme and gel filtration chromatography were performed to recovery recombinant HBsAg. 方法:采用超声与DNA酶处理,凝胶过滤柱层析的步聚回收重组HBsAg。
DEAE-Sepharose column was used to dialyze the enzyme in pH 8.0 buffer. Dialyzed enzyme specific activity was increased from 3.21U/mg to 29.21U/mg through DEAE-Sepharose, and recovery yield of activity reached 70.1%. 在pH值为8.0的条件下用SepharoseDEAEFastFlow离子交换透析酶液,比酶活从3.21U/mg提高到29.21U/mg,酶收率达70.1%。
Effect of Antioxidation Enzyme Recovery System to the Winter Resistance of Pinus Bungeana Seedlings 抗氧化酶促防御系统对白皮松幼苗抗寒性的影响
After 10 min of cerebral ischemia and a period of reperfusion with NS, the enzyme activity recovered to 72.6% that of the sham group, but the recovery of the enzyme activity was not improved by giving SY after ischemia. 脑缺血10min后给生理盐水再灌注7d,酶活性恢复至假手术组的72.6%,而缺血后给药再灌注不能加速酶活性的恢复;
The method possesses the practical value for its advantages of simple operation, short separating cycle and high enzyme recovery. 该法步骤简单、分离周期短、酶活回收率高、具有较大的实用价值。
Results: In the first method, the enzyme solution contained three proteins, enzyme recovery rate was 2.7%; 结果:方案一得到了含三条电泳条带的酶液,酶回收率达2.7%;
The specific activity of the purified enzyme increased 33.7 fold over the crude extract with 46.1% recovery. 最后得到的过氧化物酶样品纯化了33.7倍,回收率为46.1%。
Major factors considered were key enzyme induction, degradation, inactivation, recovery, and the role of energy in terms of reductive potentials. 主要影响因素包括关键酶的诱导合成、老化降解、毒性失活、自我恢复、以及能量的调节作用等。
After ischemia S-T segments were obviously elevated, activity of SDH decreased (+) and that of lactate dehydrogenase ( an important enzyme in anaerobic ( glycolysis) increased (+++), and recovery of CI and LVSWI was worse. 缺血后心电图S-T段明显抬高,SDH活性减弱(+),无氧糖酵解标志酶&乳酸脱氢酶活性增高(+++),CI和LVSWI恢复较差。
The enzyme recovery and the purification were 9 3% and 9. 2 fold, respectively. pH7.4;匀浆液加入量为体系的1%时,吐温80相平均酶活收率93%,纯化倍数9.2。
The synthesis of mesoporous molecular sieve MCM-41 and immobilizing the lipase on it by simple physical adsorption was studied in the paper. The influence on the enzyme activity and recovery under different immobilizing conditions was also studied. 本实验以自制的MCM&41为载体,利用操作简单的物理吸附法来固定脂肪酶,研究了不同固定化条件对固定化酶酶活及活性回收率等的影响。
The immobilized enzyme activity is 186.8U/cm2 relative activity is 78.69% and the recovery rate of the enzyme is above 40%. 在此条件下,固定化酶活力为186.8U/cm2,相对活力78.69%,活力回收>40%。
By comparing with each other in the enzyme recovery, optimal reaction conditions, yields of GOS and mechanical strength of the carriers, the gelatin was selected as better carrier for immobilization. 通过比较三种方法的酶活力回收、最适反应条件、GOS的得率和和载体机械强度,选择明胶作为固定化细胞的载体。
Reversed micellar extraction was relatively new separation technique which could provide high selectivity and make less inactivation of enzyme, thus being highly potential for recovery and purification of bioactive products. 反胶束萃取是一项新的分离技术,不仅具有高选择性,而且不易使酶蛋白失活,在提取纯化生物活性物质方面具有巨大的工业应用潜力。
Factors of enzyme volume and glutaraldehyde concentration have significant effects on recovery. 酶的添加量和戊二醛浓度对酶活力回收率有极显著的影响,而固定化时间对酶活力回收率的影响不大。
The PHB depolymerase was separated and purified by centrifugation, ultrafiltration, ( NH4) 2SO4 salting out and gel filtration chromatography. The specific activity of the purified enzyme was increased by 37.9 folds over crude extract, and the recovery yield was 11.81%. 发酵液经过离心、超滤浓缩、硫酸铵沉淀、层析纯化后,得PHB解聚酶,纯化倍数为37.9,回收率11.81%。
The enzyme activity recovery rate was about 33%. The stepwise purification of the enzyme had similar results as the gradient method, but was more convenient for handling large volumes of cell supernatants. 批量处理纯化重组保幼激素酯酶时酶蛋白活力回收率33%,效果与梯度分离方法相当,但简便快速,可作为大量分离纯化的第一步。
The immobilized AchE by PVA-SbQ has higher enzyme activity recovery and more than 70 percent activity was gotten after 6 months placed in room temperature. 经PVA-SbQ固定后的乙酰胆碱酯酶具有较高的活性回收率,而且在室温下放置6个月后,固化乙酰胆碱酯酶的活性仍可保持初始固化酶活性的70%以上。
After getting the best conditions of the carrier, various factors were discussed to determine the finest immobilization conditions. The enzyme activity recovery determined was 47% at the optimal condition. 在最优化制备条件下,测定了固定化酶的活性回收率为47%。